Division of Pathobiology of the Nervous System (Center for Brain Research)
Position: Associate Professor
Location: Spitalgasse 4
Phone: +43 (0)1 40160-34092
E-Mail: petra.scholze@meduniwien.ac.at
Research Area
Cysteine Loop Ligand-Gated Ion Channel Receptors, Neuropharmacology
Research Interests
Neuropharmacology, Receptors, GABA-A, Receptors, Nicotinic
Grant(s)
- Human Nicotinic Receptor Polymorphisms in Synaptic Pathology (Head)
- Involvement of GABAA receptors in modulation of neuropathic pain in animal models (Head)
- Neuronal nicotinic acetylcholine receptors dissected (Head)
- Untersuchung neuer, möglicherweise nervenspezifischer, Varianten des Organischen Kationen-Transporters OCT3 (Head)
Abstract
Both GABA-A and nAChRs are of pentameric structure, where 5 out of a set of 19 (GABA-A) or 17 (nAChR) subunits assemble to form different receptors subtypes. The functional properties of these subtypes are primarily determined by their subunit composition, and knowledge of the molecular structure is thus crucial for an understanding of receptor function in health and disease. Different receptor subtypes also display different drug binding sites, and the identification of subtype selective compounds is of great interest for both GABA-A and nACh receptors.
Techniques
- Cultivation of primary neurons (of Hippocampus or superior cervical ganglion)
- Cultivation and Transfection of HEK-293-cells
- Radioligand binding assays
- drug induced neurotransmitter release out of synaptosomes, slices and cell cultures
- molecular biology (cloning of receptor mutants)
Selected publications
- Beiranvand F, Zlabinger C, Orr-Urtreger A, Ristl R, Huck S, Scholze P (2014) Nicotinic acetylcholine receptors control acetylcholine and noradrenaline release in the rodent habenulo-interpeduncular complex Br J Pharmacol, 171 (23): 5209-5224
| Article (PDF) | - Scholze P, Koth G, Orr-Urtreger A, Huck S (2012) Subunit composition of α5-containing nicotinic receptors in the rodent habenula J Neurochem, 121(4): 551-560
| Article (PDF) | - Scholze P, Ciuraszkiewicz A, Grössl F, Orr-Urtreger A, McIntosh JM, Huck S (2011) Alpha4beta2 Nicotinic Acetylcholine Receptors n the Early Postnatal Mouse Superior Cervical Ganglion Dev. Neurobiology, 71 (5): 390-399
| Article (PDF) | - David R, Ciuraszkiewicz A, Simeone X, Orr-Urtreger A, Papke RL, McIntosh JM, Huck S, Scholze P (2010) Biochemical and functional properties of distinct nicotinic acetylcholine receptors in the superior cervical ganglion of mice with targeted deletions of nAChR subunit genes Eur J Neurosci, 6: 978-993
| Article (PDF): 1 2 | - Scholze P., Orr-Urtreger A., Changeux J.-P., McIntosh J.M. , Huck S (2007) Catecholamine outflow from mouse and rat brain slice preparations evoked by nicotinic acetylcholine receptor activation and electrical field stimulation Br J Pharmacol, 151: 414-422
| Article (PDF) |
