Ruth Drdla-Schutting
Synaptic plasticity within the central nervous system plays a crucial role in initiating and sustaining various forms of chronic pain. Our research has been predominantly centred on neurons, exploring neural mechanisms of plasticity (Science 2009, 325(5937):207-10; Science 2012, 335(6065):235-8). More recently, studies have highlighted the substantial role of glial cells, specifically astrocytes and microglia, in synaptic processes, particularly within the context of pain.
We and others have shown that glial cells are not only necessary but also sufficient for specific forms of plasticity at nociceptive synapses (Science 2016, 354(6316):1144-1148). However, our understanding of the underlying mechanisms remains limited. Consequently, we aim to elucidate the mechanisms of neuro-glia interactions related to synaptic plasticity and pain behaviour. Our focus extends to both the spinal cord and the brainstem, with an additional emphasis on exploring potential sex differences in these processes. We also aim to gain further insights into the involvement of glial cells in pain resolution.
To achieve this, we currently employ a comprehensive approach, combining in vitro and in vivo electrophysiology with chemo- and optogenetic methods. Additionally, we use calcium imaging in acute slice preparations. These techniques are complemented by a battery of behavioural tests, that allow us to assess both spontaneous and evoked pain-related behaviour in rodents.
